doiSerbia

Effect of denture base resin extracts on HeLa cells growth in vitro

Kostić Milena (Klinika za stomatologiju, Odeljenje za stomatološku protetiku, Niš)
Najman Stevo (Klinika za stomatologiju, Odeljenje za stomatološku protetiku, Niš)
Kocić Jelena S. (Medicinski fakultet, Institut za biomedicinska istraživanja, Niš)
Krunić Nebojša (Klinika za stomatologiju, Odeljenje za stomatološku protetiku, Niš)
Ajduković Zorica (Klinika za stomatologiju, Odeljenje za stomatološku protetiku, Niš)
Petrović Dimitrije (Klinika za stomatologiju, Odeljenje za stomatološku protetiku, Niš)
Anđelković Maja (Klinika za stomatologiju, Odeljenje za stomatološku protetiku, Niš)

Growth of HeLa cell culture in vitro was examined, in different concentrations of four resin materials extracts which are used for denture base making. Cell growth was evaluated through density, invert microscope counting, after 48 hours of incubation and through metabolic MTT test after 3 days. Extract was taken by incubation of material sample on 37 °C in physiological solution, for 72 hours. It is given weaker growth, reduction of adherent cells count and phenotypic changes of cells grown in presence of extracts from all examined materials. Extracts of examined materials increase number of phyllopodic extensions on dose dependent manner.

Keywords: denture, acrylate, citotoxity, HeLa cells